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Millipore
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Millipore
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DS Pharma Biomedical
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Millipore
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Janvier Labs
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Millipore
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Merck KGaA
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Merck KGaA
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Jackson Laboratory
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Journal: Neuro-Oncology
Article Title: A recombinant lentiviral PDGF-driven mouse model of proneural glioblastoma
doi: 10.1093/neuonc/nox129
Figure Lengend Snippet: Development and characterization of a gliomagenic recombinant lentiviral vector encoding shCdkn2a-PDGFB-T2A-CreERT2. (A) Vector design and (B) expression of PDGFB and CreERT2 from the vector in 293FT cells. (C) Quantitative real-time PCR analysis of Cdkn2a expression in primary mouse fibroblasts infected with the shCdkn2a virus compared with a control shRNA (2 multiplicity of infection). Mean (C) ± 1 SD of 3 independent experiments are shown. **P < 0.01.
Article Snippet: Cell Culture We grew 293FT (ThermoFisher Scientific),
Techniques: Recombinant, Plasmid Preparation, Expressing, Real-time Polymerase Chain Reaction, Infection, Virus, Control, shRNA