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Jackson Laboratory primary mouse embryo fibroblast (mef)
Development and characterization of a gliomagenic recombinant lentiviral vector encoding shCdkn2a-PDGFB-T2A-CreERT2. (A) Vector design and (B) expression of PDGFB and CreERT2 from the vector in 293FT cells. (C) Quantitative real-time PCR analysis of Cdkn2a expression in primary mouse <t>fibroblasts</t> infected with the shCdkn2a virus compared with a control shRNA (2 multiplicity of infection). Mean (C) ± 1 SD of 3 independent experiments are shown. **P < 0.01.
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Development and characterization of a gliomagenic recombinant lentiviral vector encoding shCdkn2a-PDGFB-T2A-CreERT2. (A) Vector design and (B) expression of PDGFB and CreERT2 from the vector in 293FT cells. (C) Quantitative real-time PCR analysis of Cdkn2a expression in primary mouse fibroblasts infected with the shCdkn2a virus compared with a control shRNA (2 multiplicity of infection). Mean (C) ± 1 SD of 3 independent experiments are shown. **P < 0.01.

Journal: Neuro-Oncology

Article Title: A recombinant lentiviral PDGF-driven mouse model of proneural glioblastoma

doi: 10.1093/neuonc/nox129

Figure Lengend Snippet: Development and characterization of a gliomagenic recombinant lentiviral vector encoding shCdkn2a-PDGFB-T2A-CreERT2. (A) Vector design and (B) expression of PDGFB and CreERT2 from the vector in 293FT cells. (C) Quantitative real-time PCR analysis of Cdkn2a expression in primary mouse fibroblasts infected with the shCdkn2a virus compared with a control shRNA (2 multiplicity of infection). Mean (C) ± 1 SD of 3 independent experiments are shown. **P < 0.01.

Article Snippet: Cell Culture We grew 293FT (ThermoFisher Scientific), primary mouse embryo fibroblast (MEF; isolated at e12.5 from C57BL/6J mice [Jax Stock No: 000664]), and U251 (originally obtained from American Type Culture Collection) cells on tissue culture dishes in growth media consisting of Dulbecco’s modified Eagle’s medium with L-glutamine (Corning) supplemented with 1% penicillin/streptomycin (Corning) and 10% fetal bovine serum (Corning).

Techniques: Recombinant, Plasmid Preparation, Expressing, Real-time Polymerase Chain Reaction, Infection, Virus, Control, shRNA